Net Results of Nucleolar Dynamics Cell Biology Diagrams The dynamics of the nucleolus Crit Rev Eukaryot Gene Expr. 2003;13(1):39-54. doi: 10.1615 compartments can change in response to alterations in the levels of transcription and phosphorylation and during mitosis. This review focuses on nucleolar dynamics, studied at each of the single protein, collective proteome, and subnucleolar
The dynamics of the processing nucleolar proteins was analyzed at the transition mitosis/interphase. Rapid time-lapse videomicroscopy was used to follow GFP-tagged processing proteins in the volume of the cells from metaphase to G1 (Fig. 5 ).
Assembly and disassembly of the nucleolus during the cell cycle Biology Diagrams
The dynamics of nucleolus during mitosis. The nucleolar disassembly and reassembly pathway are shown in a-c and d-i, respectively. During disassembly, (a and b) the RPA39 (blue) dissociates from the nucleoli โผ2 min before the loss of functional integrity of the NE as illustrated by the leakage of IBB (gray).
In the vast majority of eukaryotic somatic cells the nucleolus is the largest and the most dynamic nuclear domain. The canonical nucleolar function is ribosome biogenesis, which includes ribosomal DNA (rDNA) transcription, pre-rRNA processing and ribosomal subunit assembly. Furthermore, according to recent data the nucleolus and specific nucleolar proteins participate in cell cycle regulation Nucleolar dynamics throughout mitosis. Using different cell biology and biochemistry techniques 28,60,61,62 it has been documented that the nucleolar structure and biomolecular dynamics change
Nucleolar dynamics and interactions with nucleoplasm in living cells Biology Diagrams
These nucleolar functions are realized predominantly in interphase and, apparently, are abolished during mitosis, when the nucleolus disassembles. In this review, literature and our own data on the dynamics and mechanisms of the nucleolus disassembly and reassembly during mitosis in animal and plant cells are summarized. Entry into mitosis correlates with nucleolar disassembly and shutdown of ribosomal RNA (rRNA) gene (rDNA) transcription. In telophase, nucleoli reform and transcription is reactivated. The molecular mechanisms underlying the dynamics of nucleolar transcription during the cell cycle are manifold.
